Figure 2.

(A) SAMs presenting a phosphonate capture ligand for the covalent immobilization of cutinase−fibronectin fusion proteins. (B) Substrates were prepared by first treating a maleimide-terminated monolayer with a phosphonate ligand and then with a cutinase fusion protein to immobilize the FN fragments. (C) SAMDI mass spectrometry was used to confirm the masses of the ligands presented on the surface after each step.