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Representative BHK cells attached to (A) adsorbed, full length FN, (B) FN9−10, (C) FN9, (D) FN10, (E) FN9−10m, and (F) FN9m−10 and were fixed and stained for actin (left) and vinculin (middle). The overlay images on the right show the cytoskeleton in green, FAs in red, and nuclei in blue. Cells on surfaces lacking the RGD sequence (C,E) remain poorly spread and display less-defined cytoskeletons and FAs than those presenting RGD (A,B,D,F).
