Figure 2. A CEN-based plasmid containing TLC1 complemented the sensitive phenotype of type I survivors.

(A) Wild-type, pre-senescent tlc1 mutant, type I or type II strains carrying pRS316 or pRS316-TLC1 plasmid were five-fold serially diluted and spotted onto YEPD plates containing bleomycin in various concentrations. (B) Southern blot analysis of the XhoI-digested genomic DNA isolated from strains described in panel A probed to detect telomeric sequences.