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. 2009 Dec 14;4(12):e8224. doi: 10.1371/journal.pone.0008224

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Lin et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) The percentage of cells forming spontaneous Rad52-YFP foci was scored as described in Materials and Methods. (B) The percentage of Rad52-YFP foci colocalized with Rap1-Myc. Wild-type and type I cells expressing Rad52-YFP and Myc-tagged Rap1 were arrested in G1 with α-factor for 2.5 hours. Half of the culture was exposed to bleomycin for 30 min to generate DSBs. Cell treated with or without bleomycin were released into S-phase for 90 min. Before the addition of belomycin, the proportion of colocalized Rad52 in type I cells differed from that in wild-type in a statistically significant manner (P values = 0.019), and in type I cells, the percentage of colocalization prior to bleomycin treatment (black bars) also differed from that after treatment (gray bars) in a statistically significant manner (P values = 0.0232). Error bars represent standard deviations for at least three independent experiments. (C) Images of representative cells from the experiment in panel B show colocalization of Rad52 and Rap1 in type I cells. Scale bar, 2 µm. Arrowheads mark selected foci. BF, bright field.