Figure 3.

The effects of LC on HC secretion in vitro. HC_HL or HC⁷⁴⁵ were co-transfected into 293 cells along with LC expression plasmid at ratios as indicated by calcium phosphate precipitation in triplicate. Media were harvested 72 hours after transfection. (a) HC antigen was measured by heavy-chain-specific ELISA. (b) Functional FVIII was converted from activated partial thromboplastin time assay. Standard error is shown (n = 3). ELISA, enzyme-linked immunosorbent assay; FVIII, factor VIII; HC, heavy chain; LC, light chain.