Fig. 5.

Inhibition by SD0006 in RASFs of p38 kinase activation and activity, but not of ERK or JNK. RASF cells were preincubated with inhibitor for 60 min, then challenged with human recombinant IL-1β (1 ng/ml, 30 min) as described in Materials and Methods. a Phosphorylation of Hsp27 was determined by subjecting lysates to Western blotting with phospho-specific antibodies, followed by densitometry. Error bars are the standard deviation for 3 assays. b Activation states of p38, ERK, JNK, and phosphorylation of the JNK substrate, cJun, were determined by subjecting lysates to electrochemiluminescence-based ELISAs as described in Materials and Methods. Data shown are the average values of 2 assays.