Table 2.
Mini-λs and Plasmids
| Mini-λ or Plasmid | Relevant Genotype | Antibiotics Concentration (μg/ml of media) | Source or Reference |
|---|---|---|---|
| Primary Use: Express Red Functions for recombineering | |||
| mini-λ Kan (kanamycin) | contained in DH10B | 30 | 31 |
| mini-λ Tet (tetracycline) | contained in DH10B | 12.5 | 31 |
| mini-λ Cat (chloramphenicol) | contained in DH10B | 12.5 | 31 |
| mini-λ Amp (ampilcillin) | contained in DH10B | 30 | 31 |
| pSIM5 | pSC101ts gam exo bet chloramphenicol resistance (CmR) | 12.5 | 45 |
| pSIM6 | pSC101ts gam exo bet ampicillin resistance (AmpR) | 100 | 45 |
| pSIM7 | pBBR1 gam exo bet chloramphenicol resistance (CmR) | 12.5 | 45 |
| pSIM8 | pBBR1 gam exo bet ampicillin resistance (AmpR) | 100 | 45 |
| pSIM9 | pRK2 gam exo bet chloramphenicol resistance (CmR) | 12.5 | 45 |
| pSIM17 | pSC101ts gam exo bet blasticidin resistance (BsdR) | 50 | 45 |
| pSIM18 | pSC101ts gam exo bet hygromycin resistance (HygR) | 50 | 45 |
| pSIM19 | pSC101ts gam exo bet spectinomycin resistance (SpecR) | 100 | 45 |
| Primary use: Amplify galK for Counter-selection | |||
| pgalK | pBluescript SK- pEm7-galK ampicillin resistance (AmpR) | 100 | 40 |