Table 1.

Incorporation rates of unnatural triphosphates dXTP.^[a]

5'-dTAATACGACTCACTATAGGGAGA
3'-dATTATGCTGAGTGATATCCCTCTXGCTAGGTTACGGCAGGATCGC
X	kcat [min−1]	KM [µM]	kcat/KM[M−1 min−1]
d3MPy	0.42 ± 0.14	163 ± 23	2.6 × 103
d4MPy	3.2 ± 0.6	201 ± 27	1.6 × 104
d5MPy	0.34 ± 0.02	117 ± 33	2.9 × 103
d34DMPy	1.2 ± 0.2	267 ± 22	4.7 × 103
d35DMPy	0.10 ± 0.02	89 ± 19	1.2 × 103
d45DMPy	2.0 ± 0.2	49 ± 7	4.1 × 104
dQL	0.74 ± 0.05	20 ± 3	3.7 × 104
dEPy	nd[b]	nd[b]	<1.0 × 103
dAPy	nd[b]	nd[b]	<1.0 × 103
dMAPy	nd[b]	nd[b]	<1.0 × 103
dDMAPy	0.26 ± 0.02	162 ± 42	1.6 × 103

^[a]See Supporting Information for details.

^[b]Reaction was too inefficient for k_cat and K_M to be determined independently.