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. Author manuscript; available in PMC: 2009 Dec 9.
Published in final edited form as: J Mol Biol. 2006 Oct 4;365(2):513–522. doi: 10.1016/j.jmb.2006.09.088

Figure 2. All phage P22 coat subunits are competent for assembly.

Figure 2

Panel A shows densitometry profiles for partial capsids (closed circles, solid line) and in vitro assembled procapsids (open circles, dotted line) run on a 5 – 20 % linear sucrose gradient analyzed by SDS-PAGE, and Coomassie stained. Panel B represents the percent of subunits found where monomeric subunits sediment in a 5 – 20 % sucrose gradient (fractions 1 – 7) for reactions resulting in partial capsids (no salt), procapsids (salt de novo), and procapsids formed after salt was added to partial structures (salt after 20 hours). Panel C represents light scattering data, where salt (closed circles, solid line) and buffer (dotted line) was added to partial structures. The arrow shows the time of NaCl addition.