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. Author manuscript; available in PMC: 2010 Mar 1.
Published in final edited form as: Int J Oncol. 2009 Mar;34(3):729–742. doi: 10.3892/ijo_00000199

Figure 10.

Figure 10

Effect of siRNA treatment on virus-induced autophagy. No. 10 human glioma cells were transfected with 20 pMol of BECN1 (anti-beclin) siRNA and then transduced with the competent vectors as indicated: AdWT, AdRGD or CRAd-S-RGD for 72 h incubation. The cells were then examined by acridine orange staining (A) or viral E1A/beclin expression was detected by immunofluorescence (B). Inhibition of autophagosome development was measured in two independent experiments, with each experiment containing three wells per each treatment group, including controls. Data are presented as mean ± SD. *p<0.05.