Figure 4.
Promoter dependent E1A expression in human gliomas. (A) U87MG, U118MG, U373MG, A172 and No. 10 glioma cells or (B) primary glioma slices obtained from a patient were seeded in 6-well plates overnight and then infected with CRAd-S-RGD, AdRGD or AdWT. After 4 h adsorption, cells were rinsed with PBS, unbound virus was removed and fresh growth media was added. Twenty-four or 48 h post-infection, total RNA was extracted and subjected to the quantification RT-PCR. All results are presented as E1A copy number per ng RNA. *p<0.05 (statistical significance in comparison with the AdWT).