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. Author manuscript; available in PMC: 2010 Mar 1.
Published in final edited form as: Int J Oncol. 2009 Mar;34(3):729–742. doi: 10.3892/ijo_00000199

Figure 6.

Figure 6

Microarray analysis. (A) Survivin-activated adenovirus induced differential gene expression identified by oligo microarray method. Glioma cells were incubated 24 h with AdWT or CRAd-s-RGD. Total RNA was isolated and subjected to the microarray analysis. (B) Validation of microarray data by Western blotting. Total cellular proteins (50 μg) from infected glioma cells were analyzed in Western blotting with antibodies that recognize apoptotic proteins and the mitochondrial BAX proteins. BAX was not activated by CRAd-S-RGD infection, but was upregulated in AdWT-infected cells. Anti-β-actin antibody was used as a loading control. Cellular p53 and adenoviral proteins (E1A) were used to illustrate the correlation between the expression of these proteins and the downstream targets involved in apoptotic pathways.

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