Figure 7.

Exposure to BU-32 induces apoptosis in tumor cell lines. Annexin staining was conducted with the use of a kit (Annexin V--FITC Apoptosis detection kit; BD Pharmingen (San Jose, CA, USA)). The breast cancer cells were treated with 5 nM, 50 nM, and 500 nM Bortezomib and BU-32 for 24 hours. Cells were stained with annexin V--FITC and propydium iodide according to the manufacturer's protocol before analysis by flow cytometry. Results are mean ± standard deviation of three independent experiments for (a) MDA-MB-231, (b) MCF7 and (c) SKBR3 cell lines.