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. 2009 Sep 28;37(21):7239–7257. doi: 10.1093/nar/gkp668

Figure 6.

Figure 6.

RsaE ortholog is expressed in B. subtilis. (A) Secondary structure of B. subtilis RsaE derived from the structural alignment shown in Figure 2. The C-rich conserved boxes are shown in black. (B) Expression of B. subtilis RsaE: (left) Primer extension was used to map the 5′ start of S. aureus and B. subtilis RsaE. The coordinates of the RNA are 1232743–1232852 corresponding to Bacillus subtilis subsp. subtilis strain 168 (NC_000964.2). The RNA has been referred in GenBank with the accession number GQ403626. Lanes U, G, C and A: sequencing ladders. (Right) Northern experiment performed on S. aureus and B. subtilis RsaE. Both experiments were carried out on total RNA extracts prepared at the exponential (E) and stationary (S) phases. (C) Potential base pairings between B. subtilis RsaE and mRNAs encoding proteins involved in carbohydrate metabolism and sugar utilization. The Shine‐Dalgarno (SD) sequence is in red.