Table 2.
Strains and plasmids
| Name | Revelant characteristics | Reference or source | |
|---|---|---|---|
| Escherichia coli | DH5α, cloning strain | Laboratory stock | |
| Bacillus subtilis | Wild type strain | ATCC6051 | |
| S. aureus strains | |||
| RN4220 | restriction- mutant of 8325‐4 | (105) | |
| RN6390 | derivative of 8325-4, agr positive | (106) | |
| RN6911 | RN6390: Δagr region::tetM | (107) | |
| WA400 | 8325-4: ΔrnaIII region::cat86 | (36) | |
| COL | Methicillin-resistant laboratory strain | (108) | |
| Newman | Wild type strain | (109) | |
| SH1000 | rsbU+ strain derivative of 8325-4 | (56) | |
| RN1HG | rsbU+ strain derivative of RN1 | (57) | |
| LUG774 | RN6390: Δrnc region::cat86 | (14) | |
| LUG911 | RN6390: Δhfq region::cat86 | (9) | |
| LUG1160 | RN6390: Δrot region::cat86 | This work | |
| LUG1397 | RN4220/pLUG766 | This work | |
| LUG1408 | RN6390/pLUG766 | This work | |
| LUG1430 | RN6390: ΔrsaE:: aphA-3 | This work | |
| E. coli plasmids | |||
| pUT7_RsaG | T7 promoter/RsaG | This work | |
| pUC18_T7_RsaA | T7 promoter/RsaA | This work | |
| pUC18_T7_RsaH | T7 promoter/RsaH | This work | |
| pUC18_T7_RsaE | T7 promoter/RsaE | This work | |
| pUC18_T7_oppB | T7 promoter/5′-region of oppB (nt −182 to +182, +1 being the A of AUG codon) | This work | |
| pUC18_T7_SA0873 | T7 promoter/5′-region of SA0873 (nt −22 to +128) | This work | |
| E. coli-staphylococcal shuttle plasmids | |||
| pMAD | Thermosensitive origin of replication, constitutively expressed βgalB gene | (37) | |
| pLUG766 | pMAD derivative for deletion/ replacement of S. aureus rsaE gene | This work | |