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Mass chromatogram and spectra of synthetic siRNAs (21-mer). (a) Base peak chromatogram of two complementary siRNAs (50 fmol each). (b and c) The MS spectra of multiply charged (b) GACUCCUAUGCCAACUACUdTdT-OH and (c) AGUAGUUGGCAUAGGAGUCdTdT-OH ions. Insets, the enlarged spectra of the 9− ions.
