Table 1.

Assignments of oligonucleotide fragments found in the RNase digest of the yeast tRNA^Phe-1 preparation

Peak number	Observed			Theoretical	Δppm	tRNAa	Residue numbersa	Sequencea
	m/z	Charge	Molecular mass	Molecular mass
1	649.0779	−1	650.0858	650.0882	3.7	Phe1, Phe2	2–3	CG>p
2	960.1170	−1	961.1249	961.1284	3.6	Phe1, Phe2	16–18	DDG>p
3	650.0623	−1	651.0702	651.0722	3.1	Phe1, Phe2	52–53	UG>p
4	590.1003	−2	1182.2164	1182.2195	2.6	Phe1, Phe2	72–75	CACC-OH
5	673.0939	−1	674.1018	674.0995	−3.4	Phe1, Phe2	44–45	AG>p
6	794.0939	−2	1590.2036	1590.2065	1.8	Phe1, Phe2	11–15	CΨCAG>p
7	637.0674	−2	1276.1506	1276.1538	2.5	Phe1, Phe2	54–57	TΨCG>p
8	754.6255	−2	1511.2668	1511.2718	3.3	Phe1, Phe2	72–76	CACCA-OH
9	649.0736	−2	1300.1630	1300.1651	1.6	Lys2	7–10	UUAm2G>p
10	969.1186	−2	1940.2530	1940.2576	2.4	Phe1, Phe2	46–51	m7GUCm5CUG>p
11	980.1395	−2	1962.2948	1962.3009	3.1	Phe1, Phe2	25–30	Cm2,2GCCAG>p
12	1282.6711	−2	2567.3580	2567.3677	3.8	Phe1, Phe2	58–65	m1AUCCACAG>p
13	966.1183	−2	1934.2524	1934.2584	3.1	Phe2	5–10	ACUUAm2G>p
14	966.6118	−2	1935.2394	1935.2424	1.6	Phe1	5–10	AUUUAm2G>p
14	959.1121	−2	1920.2400	1920.2428	1.5	Phe1	66–71	AAUUCG>p
15	1463.6897	−2	2929.3952	2929.4075	4.2	Tyr	37–45	ΨA(i6A)AΨCUUG>p
16	1381.2057	−3	4146.6408	4146.6543	3.3	Phe1, Phe2	31–42	ACmUGmAAyWAΨm5CUG>p

^aAccording to the MODMICS database²⁷ (http://genesilico.pl/modomics).