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. 2009 Sep 18;37(21):7039–7046. doi: 10.1093/nar/gkp749

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© The Author(s) 2009. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — Single cell LOI distributions of PLAGL1 and ZNF331 in human trophoblasts. Histograms show the percentage of cells exhibiting a given LOI. In order to present data with positive and negative values of ΔCp based on a single allele and limit LOI to 0–100%, data with negative ΔCp were calculated using the alternative allele. (A–B) LOI for primary PLAGL1 homozygous trophoblast controls. (C) LOI for ZNF331 heterozygotes (scale limited to 35–100% LOI). (D–G) LOI of PLAGL1 heterozygotes with significant LOI (35–100%). (D) Primary cytotrophoblasts; (E) Untreated HTR8 cells. (F) HTR8 cells treated 1 day with AZA. (G) HTR8 cells treated 2 days with AZA. The Fisher’s exact test was used to determine significance of the percentage of cells exhibiting LOI compared with the untreated control. (*P < 0.05; **P < 0.01).