Figure 2.

Sequence analysis of the proximal region of SMYD1 gene promoter and mapping of promoter activity in C2C12 cells. (A) Conserved binding sites of myogenic regulators in SMYD1 gene, which include the basic helix–loop–helix proteins (E-box), MEF2, and serum response factor (SRF) are indicated with relative positions. The sequence of each binding site is listed along with homologous motifs from other species (human, mouse and rat). (B) Deletion analysis of SMYD1 gene promoter fragments with luciferase reporter assays. C2C12 cells were transiently transfected with various SMYD1 promoter deletion constructs and the pGL3-Basic plasmids were transfected as a negative control. Promoter-driven luciferase activity was determined as described in ‘Materials and Methods’ section.