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. 2009 Sep 25;37(21):7059–7071. doi: 10.1093/nar/gkp773

Figure 3.

Figure 3.

Regulation of Smyd1 expression by SRF and myogenin. (A) Smyd1 expression during C2C12 cell differentiation. C2C12 myoblasts were cultured in proliferation medium to full confluence and induced to differentiate for indicated days by culturing in differentiation medium. Endogenous protein levels of Smyd1, myogenin and SRF were detected by western blot analysis (actin as a protein loading control). (B) C2C12 cells were transfected with pcDNA3.1 and pcDNA3.1-SRF, respectively. After 7 days of G418 selection, cells were pooled and cultured in differentiation medium for 2 days. Protein extracts were subjected to immunoblot analysis using anti-Smyd1 and anti-SRF antibodies, respectively. (C) C2C12 cells were transfected with pEMSV and pEMSV-myogenin respectively. After 7 days of G418 selection, cells were pooled and cultured in differentiation medium for 2 days. The protein levels of Smyd1 and myogenin were detected by western blotting with specific antibodies, respectively.