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. 2009 Sep 25;37(21):7059–7071. doi: 10.1093/nar/gkp773

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© The Author(s) 2009. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — Expression of Smyd1 in SRF null ES cells and the SRF cardiac-conditional knockout embryo. (A) Reduced expression of Smyd1 was revealed by in situ hybridization in the SRF Nkx2.5-cre cko embryo (left panel, 8.5 dpc) and SRF αMHC-cre cko (right panel, 10.5 dpc) embryo compared to controls. The arrow: atrium, black arrow head: ventricle; red arrow head: outflow tract. Scale bar: 100 μm. (B) Rescue of Smyd1 expression in Srf-null ES cells with recombinant wild-type SRF. Immunoblot showing the expression of SRF protein in Srf-null ES cells infected with SRF-lentiviruses comparing with wild type and Srf+/− ES cells (left panel). Smyd1 expression was induced by SRF expression during Srf^−/− ES cell differentiation measured by qRT-PCR. Significant induction of Smyd1 by SRF expression was observed by Days 8, 10, 12 of embryoid body differentiation (right panel).