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. 2009 Sep 22;37(21):7072–7084. doi: 10.1093/nar/gkp777

Figure 4.

Figure 4.

PCAF inhibition is not competitive with ATP nor with substrate histone H1 or cyclin A. Data from kinetic assays (see ‘Materials and Methods’ section) were analysed by double reciprocal plots. (A) Inhibition of cyclin A/cdk2 activity by PCAF at different ATP concentrations. The assay consisted in the incubation of 2 µg of substrate histone H1 with 400 nM cyclin A/cdk2 in the presence (square) or absence (triangle) of 800 nM PCAF. (B) Inhibition of cyclin A/cdk2 activity by PCAF at different histone H1 concentrations. The assay consisted in the incubation of 12.5 µM ATP with 400 nM cyclin A/cdk2 in the presence (square) or absence (triangle) of 800 nM PCAF. (C) Inhibition of cdk2 activity by PCAF at different cyclin A concentrations. The assay consisted in the incubation of 400 nM cdk2 (control) or 400 nM cdk2 plus 800 nM PCAF (+PCAF) with increasing concentrations of cyclin A in the presence of 12.5 µM ATP and 2 µg of substrate histone H1.