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Nucleotide dependence of DNA cleavage by LlaGI. Error bars represent standard errors from at least two repeat experiments. pHH-3 was incubated with excess LlaGI for 10 min in the absence or presence of 4 mM nucleotide, as indicated. Reaction products were separated by agarose gel electrophoresis and the extent of DNA cleavage quantified (‘Materials and Methods’ section).
