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1D 1H spectra of G-quartet DNA and selected gC 30-mer DNA oligonucleotides. A portion of the 1D 1H spectra (9–14 p.p.m.) collected on 0.5 mM G4 DNA [TTGGGGTT]4, gC 191–220, gC 91–120, gC 31–60, gC 11–40 and gC 1–30 at 25°C in 50 mM Tris pH 8, 150 mM KCl and 10% D2O. Spectra were scaled using a resolved H1’ ribose signal. Asterisks (*) indicate prominent peaks detected in gC 191–220 and gC 31–60 sequences, two sequences that were not shifted by the ICP27 in EMSA experiments (Figure 2).
