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. 2009 Oct 22;284(51):35287–35296. doi: 10.1074/jbc.M109.050120

FIGURE 2.

FIGURE 2.

RhoE does not affect mTOR/S6K signaling. A, RhoE-3T3 cells were starved for 24 h in 0. 5% FCS-containing medium in the presence (+Tet) or absence (−Tet) of tetracycline, stimulated for 30 min with 10% FCS alone or together with rapamycin (20 nm, preincubated for 30 min) in the presence or absence of tetracycline, and harvested. The expression levels of the indicated proteins were analyzed by Western blotting with specific antibodies. B, representation of the quantified P-S6 and p-4EBP1 (Ser-65 and Thr-37/46) levels in control (+Tet) and RhoE-expressing (−Tet) cells. C, RhoE-3T3 cells were starved for 24 h in 0.5% FCS-containing medium in the presence (+Tet) or absence (−Tet) of tetracycline and were stimulated with 10% FCS alone, in the presence (+Tet) or absence (−Tet) of tetracycline, or together with rapamycin (20 nm) in the presence of tetracycline (rapamycin). Cell size was measured at the indicated times and shown as the percentage in cell size increase relative to cell size at 0 h. Results represent the mean values from three independent experiments, each conducted in duplicate.