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. 2009 Oct 28;284(51):35297–35307. doi: 10.1074/jbc.M109.040014

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© 2009 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 4. — Effects of IscA1 knockdown on cytosolic and mitochondrial aconitase. HeLa cells were transfected with the indicated siRNAs. A–E, cytosolic or mitochondrial extracts were assayed for aconitase, succinate dehydrogenase (SDH), lactate dehydrogenase (LDH), or citrate synthase (CS) activity as indicated. Shown are the means ± S.D., n = 3. *, p < 0.01; **, p < 0.05. F, total cellular extracts (20 μg) were analyzed by Western blotting using antibodies against ferritin-H, IRP1, IRP2, or β-tubulin. G, HeLa cells were transfected with the indicated siRNAs and counted by trypan blue exclusion after 9 days. Shown are the means ± S.D., n = 3. **, p < 0.05. H, total cellular extracts (20 μg) were analyzed by Western blotting using antibodies against IscA1, IOP1, or β-tubulin. CON, control.