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. 2009 Oct 28;284(51):35297–35307. doi: 10.1074/jbc.M109.040014

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© 2009 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 5. — Effects of IscA1 knockdown on recovery of aconitase activity from DFO and hydrogen peroxide treatments. HeLa cells were transfected with the indicated siRNAs. A and B, 8 days after the initial transfection, some cells were treated with 100 μm DFO for 16 h and then refed with media lacking DFO for the indicated times. C and D, 9 days after the initial transfection, some cells were treated with 200 μm H₂O₂ for 30 min and then refed with media lacking H₂O₂ for the indicated times. Cytosolic (A and C) or mitochondrial (B and D) extracts were assayed for aconitase activity. Shown are the means ± S.D., n = 3. *, p < 0.01; **, p < 0.05.