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. 2009 Oct 7;284(51):35325–35337. doi: 10.1074/jbc.M109.035949

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© 2009 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 2. — MMC inhibits ubiquitylation of cyclin E. A, Northern blot showing that MMC does not affect transcript levels of cyclin E. HeLa cells were synchronized by double-thymidine block and analyzed at the indicated times after release into normal medium with or without MMC. B, immunoblot analysis showing that MMC prevents ubiquitylation of cyclin E. HeLa cells transiently expressing HA-ubiquitin (HA-Ub) were synchronized and released into medium with or without MMC. Lysates were subjected to IP with anti-HA and immunoblotted for cyclin E (left panel). Right panel shows a loading control. C, immunoblot analysis showing stabilization of cyclin E from the experiment in B.