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. 2009 Oct 22;284(51):35390–35402. doi: 10.1074/jbc.M109.064147

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© 2009 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 9. — MS² determination of the site of initial radical formation in the IgG1. The IgG1 was incubated at 25 °C in the presence of H₂O₂ (20 mm) and DMPO (100 mm) at pH 5.2. The sample was buffer-exchanged, digested with Lys-C, and analyzed by RP-HPLC-MS². Hinge peptide H12 (2,789 Da, corresponding to the mass with one Cys modified by IAA) was found modified by the DMPO adduct (2,902 Da). The MS² spectrum of the 1,451.98-Da ion (2+) showed the DMPO adduct to be located at Cys²³¹. For clarity, not all identified fragment ions are labeled.