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. 2009 Oct 22;284(51):35403–35411. doi: 10.1074/jbc.M109.059501

FIGURE 3.

FIGURE 3.

IRAK-1 mediates LPS-induced activation of p65 and C/EBPβ/δ. A, WT and IRAK1−/− BMDM cells were treated with 100 ng/ml LPS for 2 h. Equal amounts of total cell lysates were resolved on SDS-PAGE and blotted with antibodies specific for C/EBPδ and C/EBPβ. Data are representative of three independent experiments. B, putative binding sites of transcription factors within the proximal promoter of murine Nox-1. 1+ denotes the transcription start site. C, decreased binding of multiple transcription factors to the proximal promoter of murine Nox-1 in IRAK1−/− BMDMs. The BMDMs were either uninduced or induced with 100 ng/ml LPS for 2 h and subjected to chromatin immunoprecipitation (CHIP) assay using the indicated antibodies and primers specific to the proximal promoter of murine Nox-1. Data are representative of three independent experiments.