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. 2009 Oct 22;284(51):35403–35411. doi: 10.1074/jbc.M109.059501

FIGURE 6.

FIGURE 6.

IRAK-1 is required for LPS-mediated suppression of GPX3 and catalase. A, effect of LPS on GPX3 and catalase expression in WT and IRAK1−/− BMDM cells. The cells were stimulated with LPS (100 ng/ml) for the indicated time points. After stimulation, the mRNA levels of GPX3 and catalase were analyzed using real time RT-PCR. Each data point represents the mean ± S.D. of at least three independent experiments. *, p < 0.05, compared with control. B, protein levels of catalase were measured after LPS stimulation in WT and IRAK1−/− BMDMs by Western blot. The same blots were probed with β-actin as the loading control. C, effect of LPS on GPX3 and catalase activity in WT and IRAK1−/− BMDM cells. The cells were stimulated with LPS overnight, and the GPX3 and catalase activities were analyzed in the cell lysate using the Cayman kit. Data are representative of three independent experiments.