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. 2009 Oct 26;284(51):35556–35563. doi: 10.1074/jbc.M109.063339

FIGURE 6.

FIGURE 6.

Effect of VCAM-1 on growth regulation of cells. A, Western blot (WB) analysis of VCAM-1 in its knockdown Balb/3T3 cells is shown. Cell lysates from mock-transfected (lane 1) and VCAM-1-knockdown cells (lane 2) were analyzed by Western blot analysis with an anti-VCAM-1 antibody. B, shown is a growth curve of VCAM-1-knockdown cells (open circle) and mock-transfected cells (closed circle). Cells were cultured (2 × 104 cells/6-well plate), and their numbers were counted with a Coulter counter every 24 h. The results are shown as the mean ± S.D. of triplicate experiments. C, shown is the effect of galectin-3 on growth of VCAM-1-knockdown cells. VCAM-1-knockdown and mock-transfected cells cultured in Dulbecco's modified Eagle's medium containing 2.5% fetal calf serum (3 × 104 cells/24-well plate) were treated with 5 μm recombinant galectin-3 or PBS for 20 h and then counted by a Coulter counter. The results are shown as the mean ± S.D. of triplicate experiments.