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. 2009 Oct 26;284(51):35580–35587. doi: 10.1074/jbc.M109.060905

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© 2009 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 2. — HSF-1 is required for efficient nuclear export of DAF-16 after heat shock. A, DAF-16::GFP distribution was observed in daf-16::gfp animals grown on vector RNAi at 20 °C, immediately after heat shock at 35 °C for 1 h (HS), after recovery for 6 h (6hR), and after recovery for 24h (24hR). B, DAF-16::GFP distribution was observed in daf-16::gfp animals grown on hsf-1 RNAi at 20 °C, immediately after heat shock at 35 °C for 1 h (HS), after recovery for 6 h (6hR), and after recovery for 24 h (24hR). C, table showing the percentage of vector or hsf-1 RNAi adult animals with nuclear, intermediate, or cytosolic DAF-16::GFP after recovery for 6 and 24 h. Shown is a representative assay of more than six independent experiments. n = 50–70 nematodes per condition.