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. 2009 Oct 26;284(51):35580–35587. doi: 10.1074/jbc.M109.060905

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© 2009 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 3. — RNAi inhibition of hsp-1 delays nuclear export of DAF16::GFP after heat shock. A, DAF-16::GFP distribution was observed in daf-16::gfp animals grown on vector RNAi at 20 °C, immediately after heat shock at 35 °C for 1 h (HS), after recovery for 6 h (6hR), and after recovery for 24 h (24hR). B, DAF-16::GFP distribution was observed in daf-16::gfp animals grown on hsp-1 RNAi at 20 °C, immediately after heat shock at 35 °C for 1 h (HS), after recovery for 6 h (6hR), and after recovery for 24 h (24hR). C, DAF-16::GFP distribution was observed in daf-16::gfp L3 larvae grown on vector or hsp-1 RNAi at 20 °C. D, table showing the percentage of vector or hsp-1 RNAi adult animals with cytosolic, intermediate, or nuclear DAF-16::GFP after recovery for 6 and 24 h. Shown is a representative assay of more than three independent experiments. n = 100–110 nematodes per condition.