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. 2009 Oct 9;284(51):35612–35620. doi: 10.1074/jbc.M109.061077

FIGURE 2.

FIGURE 2.

Stop/go folding of the major 2-disulfide intermediates of LDTI. IIa, IIb, and IIc were isolated, lyophilized, and dissolved in the absence (Control −) or presence of oxidizing agent (GSSG) to reinitiate the folding reaction. Folding intermediates were subsequently trapped by acidification over time and analyzed by RP-HPLC.