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NMR characterization of intermediate I. A, overlay of the NOESY spectra of native LDTI (black) and its intermediate I (red). Interstrand NOEs are shown in dashed lines. B, ball-and-stick representation of the three-stranded antiparallel β-sheet (β1, gray; β2, cyan; and β3, yellow), where atoms Hα, HN, and O are represented by a sphere colored in black, blue, and red, respectively. The observed NOEs in the two-dimensional NOESY spectrum of intermediate I are indicated by dashed lines.
