Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
TG2 blocks the binding of Rac1 to Bcr. A, Xpress-tagged Bcr WT or Bcr R1090A (RA) was co-transfected with TG2. After IP with TG2 antibodies, interactions were visualized with Xpress or TG2 antibodies. B, bacterially purified GST-BcrGAP or GST (25 pmol each) was incubated with GTP-loaded Rac1 (25 pmol) or His6-tagged TG2 (+, 12.5 pmol; ++, 25 pmol) as indicated and complex formation was measured by glutathione-agarose PD and Western blot analysis to detect Rac1 and TG2.
