FIGURE 1.

ENaC and ENaC transcriptional regulators are expressed and regulated by aldosterone in HEK 293T cells. A, total RNA was isolated from 293T cells cultured in Dulbecco's modified Eagle's medium plus 10% fetal bovine serum and analyzed by RT-PCR in the absence (-) or presence (+) of reverse transcriptase for expression of the genes indicated. B–D, total RNA isolated from 293T cells treated for 24 h with vehicle (−Aldo) or 1 μm aldosterone (+Aldo) was analyzed by real-time RT-qPCR and examined for expression of ENaC subunits (B), ENaC transcriptional regulators (C), or CTGF and preproendothelin-1 (Endo) (D). The mRNA level of each gene was normalized against β-actin mRNA, which was invariant as measured by real-time PCR and set to 1 in the vehicle-treated cells. In all cases *, p < 0.05 versus −Aldo. n = 3.