FIGURE 6.

AF17 and Sgk1 colocalize and synergistically activate the α-ENaC promoter. A, as in Fig. 3A, representative images of confocal microscopy demonstrate colocalization of Sgk1 with AF9 as control or with AF17 in 293T cells. B, luciferase assay indicates a synergistic effect of AF17 and Sgk1 on α-ENaC promoter. 293T cells transfected with an α-ENaC promoter luciferase construct along with pCDNA3.1 vector (Vec) or its derivatives encoding AF17 or Sgk1 were analyzed as in Fig. 4D. *, p < 0.05 versus Vec.