FIGURE 7.

Dot1a, AF9, and AF17 differentially regulate mRNA expression of ENaC and other target genes. As in Fig. 1B, 293T cells were transiently transfected with pCDNA3.1 (Vec) or its derivatives expressing Dot1a, AF9, AF17, or Dot1a/AF17. Total RNA from these cells was analyzed by real-time RT-qPCR for expression of ENaC subunits (A), ENaC regulators (B), or CTGF and preproendothelin (Endo) (C). The relative abundance of mRNA of each gene was set to 1 in vector-transfected cells and used for comparison. n = 3. *, p < 0.05 versus vector for each gene.