FIGURE 5.

rpt5-K228R and rpt6-K195R point mutants alter SAGA targeting to the GAL1 UAS. A and B, shown is analysis of Gal4p and SAGA recruitment to the GAL1 UAS in rpt5-K228R and rpt6-K195R point mutants. The wild type (WT) and mutant strains were grown and cross-linked as in Fig. 2A. Immunoprecipitation was performed as in Fig. 2A. C, growth analysis is shown of rpt5-K228R and rpt6-K195R point mutants and wild type strains after induction in YPG. The wild type and mutant strains were grown in YPR up to an A₆₀₀ of 0.9 and then transferred to YPG for induction. The A₆₀₀ of both the wild type and mutant cells were measured at different induction time points. D, growth analysis is shown of rpt5-K228R, rpt6-K195R, and wild type strains in YPD. The wild type and mutant strains were grown in YPD up to an A₆₀₀ of ∼0.2, and then the A₆₀₀ of the wild type and mutant cells was measured for the next 7 h.