FIGURE 1.

The caffeine sensitivities of the aup1Δ and rtg2Δ are both suppressed by leucine prototrophy. Wild type (WT) (HAY75), aup1Δ (HAY809), and rtg2Δ (DJY2) were grown to stationary phase in SD medium, serially diluted (from an initial concentration of 6 × 10⁶ cells/ml) in 1:10 steps, and spotted onto YPD + caffeine plates (4 mm). Plates were incubated at 26 °C and imaged. Bottom row of plates, replicate aliquots were serially diluted (from an initial concentration of 6 × 10⁶ cells/ml) in 1:10 steps and spotted onto control YPD plates to demonstrate equivalent growth potential in the absence of caffeine.