Table 1. PCR Primers.
| Entrez Gene ID | Gene | Primers (5′-3′) |
|---|---|---|
| 2044 |
Eph-A5 |
F: ACCGATGAACCTCCCAAAAT |
| R: TAGCCTGCTGCTGTACGTG | ||
| 285220 |
Eph-A6 |
F: TGGGATGCCATCACTGAAAT |
| R: CAAGAGGAACCAGCCAATCT | ||
| 2045 |
Eph-A7 |
F: GATCCCAGAGGCTCTTTGC |
| R: TTGATGGACAGCTCTATTTGG | ||
| 2047 |
Eph-B1 |
F: AGCTGCTCCCGCTGTGAC |
| R: TGCTCCGATGACCTCTTCA | ||
| 1942 |
ephrin-A1 |
F: TCCGGAATGAGGACTACACC |
| R: AGTGCCTGTCCCTCTCTTCA | ||
| 1943 |
ephrin-A2 |
F: GCTGCTGCTCCTGCTGTTAC |
| R: CTCGCCGTTGACCATGTA | ||
| 1944 |
ephrin-A3 |
F: CCTGCACTGGAAGTGTCTGA |
| R: ACCAGGAGTCAGGGAAAGGT | ||
| 1945 |
ephrin-A4 |
F: TGGAGAGAGTGGCACATCAG |
| R: GGAGGGCAACAAAAAGATGA | ||
| 1946 |
ephrin-A5 |
F: GATGTGTGTGTTCAGCCAGG |
| R: AGGGAGGCAGGAACAAGTTT | ||
| 1948 | ephrin-B2 | F: TAACCAGGAGGGAGGGGTGT |
| R: GCCGAATGCTACAAGACTAGG |
PCR of several A-class Ephs and ephrins as well as Eph-B1 and ephrin-B2 was run using cDNA from four fetal human retinas of 17–20 WG. The PCR products for Eph-A5, -A6, -A7, -B1 and ephrin-A1, -A4, and -B2 were then used to generate DIG-labeled riboprobes for in situ hybridization.