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. 2009 Aug 7;85(1):155–166. doi: 10.1093/cvr/cvp275

Figure 1.

Figure 1

Endogenous generation of OA-NO2 following ischaemia and reperfusion. (A) Tissue concentration of OA-NO2 in hearts from mice undergoing 30 min of ischaemia and 30 min of reperfusion, ischaemia without reperfusion or sham surgery. (B–D) Characterization of OA-NO2 in heart tissue from mice subjected to I/R injury. The chromatograms show co-elution of OA-NO2 extracted from heart tissue with synthetic OA-NO2 (C) and the internal standard (D). (E) The table shows the peak area ratios corresponding to the two main products of OA-NO2 collision induced dissociation: m/z 326/279 (neutral loss of HNO2) and m/z 326/46 (formation of NO2−), further confirming the identity of OA-NO2 extracted from the heart tissue.