Figure 4.

Effect of OA-NO₂ on infarct size and left ventricular function following ischaemia and reperfusion. (A) Representative images of hearts from vehicle- or OA-NO₂-treated animals stained with Evan's blue dye. (B) OA-NO₂ treatment at reperfusion, 15 min or 3 days prior to reperfusion lead to a significant reduction in infarct area/area at risk ratio when compared with vehicle-treated animals (*P < 0.01 vs. vehicle). Pretreatment with pyrrolidinedithiocarbamate (PDTC) abolished the effect of OA-NO₂. (C) Infarct area to left ventricular (IA/LV) areas reflected the results observed for the IA/AAR ratio (*P < 0.01 vs. vehicle). (D) No difference in AAR/LV ratio was observed between the different treatment groups. (E) OA-NO₂ treatment resulted in a significantly improved left ventricular function after I/R, as assessed by transthoracic M-mode echocardiography (*P < 0.01).