Figure 7.

Effect of OA-NO₂ on leukocyte infiltration and systemic inflammation following ischaemia and reperfusion. (A) Immunofluorescent staining for ICAM-1 (red) and CD31 (green) in vessels of OA-NO₂ (right panel) and vehicle-treated animals (left panel). OA-NO₂-treated animals showed a marked reduction in ICAM-1 immunoreactivity. (B) Neutrophil accumulation in infarcted zone of myocardium. Immunofluorescent staining for neutrophil-specific Ly-6-G revealed increased neutrophil infiltration in vehicle-treated mice. (C) Determination of MPO tissue levels confirmed increased neutrophil infiltration in vehicle-treated animals. (D) Serum TNFα levels were significantly reduced in OA-NO₂ treated animals 6 h after reperfusion. (E) Serum IL-6 levels underwent significant reduction in OA-NO₂-treated mice.