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Association of p300 and U-STAT3. Lysates from HEK-AT1R, Flag-Y705F-STAT3, and Flag-WT-STAT3 cells (A–C) or heart tissue (C) were used for co-immunoprecipitation assay as indicated. (C) Cell lysates from WT-STAT3 stimulated with (+) or without (−) Ang II were used as positive control; mIgG: mouse normal IgG, was used as a negative control. Y705F-STAT3: Flag-Y705F-STAT3 and HA-AT1R double transfected HEK293 cells; WT-STAT3: Flag-WT-STAT3 and HA-AT1R double transfected HEK293 cells.
