Table 1.
List of primers/probes used for real-time qPCR.
| Primer name | Sequence (5′–3′) | GenBank Accession number or source |
|---|---|---|
| IL-8 forward | TTCCAAGCTGGCTGTTGCT | X78306 |
| IL-8 reverse | TTGACAGAACTGCAGCTTCACA | |
| IL-8 probe | FAM-CCGCTTTCCTGCTCTCTGCAGCTC-TAMRA | |
| RANTES forward | TGCTTCTGCCTCCCCATATG | Based on alignment of conserved sequences between human (NM_002985) and bovine (NM_175827) RANTES |
| RANTES reverse | GGGCGGGAGATATAGGCAAA | |
| RANTES probe | FAM-CACCACGCCCTGCT-MGBNFQ | |
| MCP-1 forward | GCTGTGATTTTCAAGACCATCCT | Based on conserved sequences reported in [28] |
| MCP-1 reverse | GGCGTCCTGGACCCATTT | |
| MCP-1 probe | FAM-AAAGAGTTTTGTGCAGACCCCAACC-TAMRA | |
| IFN-γ forward | TGGAGGACTTCAAAAGGCTGAT | NM_001009803 |
| IFN-γ reverse | GATGGCTTTGCGCTGGAT | |
| IFN-γ probe | FAM-CAAATTCCGGTGGATGATCTGC-TAMRA | |
| IL-6 forward | GCTGCTCCTGGTGATGACTTC | NM_001009392 |
| IL-6 reverse | GGTGGTGTCATTTTTGAAATCTTCT | |
| IL-6 probe | FAM-CTTTCCCTACCCCGGGTCCCCTG-MBGNFQ | |
| TNF-α forward | CAACCTGGGACACCCAGAAT | NM_001024860 |
| TNF-α reverse | TCTCAAGGAACGTTGCGAAGT | |
| TNF-α probe | FAM-CAAGGGCCAGGGTTCTTACCGGAA-TAMRA | |
| IFN-β forward | TGGTTCTCCTGCTGTGTTTCTC | NM_174350 |
| IFN-β reverse | CGTTGTTGGAATCGAAGCAA | |
| IFN-β probe | FAM-ACCACAGCTCTTTCCAGGAGCTACA-TAMRA | |
| GMCSF forward | CCCTGGCAGCTTGTGGAT | EU287484 |
| GMCSF reverse | GTGTCAGTGCTGTCGTTCAGAAG | |
| GMCSF probe | FAM-CCATCAAGGAGGCCCT-MGBNFQ | |
| C3 forward | GGACACCAGGGAGAAAGATGTG | AF038130 |
| C3 reverse | CGTGCAGCGCGATCAG | |
| C3 probe | FAM-CCCTTACAGCCTTTG-MGBNFQ | |
| CFB forward | GGCCCCCTGATTATTCACAA | EF446375 |
| CFB reverse | CAGCTGATCACGCCAACTTG | |
| CFB probe | FAM-AGGAGCCGCTTCAT-MGBNFQ | |
| CFH forward | CGGTGTCAGGCCTACTATGAACT | EU888587 |
| CFH reverse | GGTTCTGACCACTCTCCATTCC | |
| CFH probe | FAM-AGGGAAACAGAAACGTAGTAT-MGBNFQ | |
| TLR4 forward | CCATCGCCGCCAATATCA | DQ922636 |
| TLR4 reverse | TGGGACACCACGACAATAACC | |
| TLR4 probe | FAM-CCAGGAGGGTTTCCACAAAAGCCGT-TAMRA | |
| TGF-β forward | TGT GTT CGT CAG CTC TAC ATT GAC | NM_001009400 |
| TGF-β reverse | TAG CCC TTG GGT TCG TGA AT | |
| TGF-β probe | FAM-TCC AGC CCA GGT CCT TCC GGA-TAMRA | |
| IL-10 forward | GTCGGAAATGATCCAGTTTTACCT | Z29362 |
| IL-10 reverse | GTCAGGCCCATGGTTCTCA | |
| IL-10 probe | FAM-AGGAGGTGATGCCACAGG-MGBNFQ | |
| SP-A forward | TGACCCTTATGCTCCTCTGGAT | AF076633 |
| SP-A reverse | GGGCTTCCAAGACAAACTTCCT | |
| SP-A probe | FAM-TGGCTTCTGGCCTCGAGTGCG-TAMRA | |
| SP-D forward | ACGTTCTGCAGCTGAGAAT | AJ133002 |
| SP-D reverse | TCGGTCATGCTCAGGAAAGC | |
| SP-D probe | FAM-TTGACTCAGCTGGCCACAGCCCAGAACA-TAMRA | |
| SAA forward | GGGACGAGGTACGGAAGGAT | EU366476 |
| SAA reverse | TCCGGCCCCATTCGTT | |
| SAA probe | FAM-CGAAGGCCGACCAGT-MGBNFQ | |
| SMAP29 forward | GGCCCAACTGTTCTCCGAAT | L46854 |
| SMAP29 reverse | GCAGACCCTTAGGACTCTTTCCT | |
| SMAP29 probe | FAM-ATCAGAATAGCTGGGTGAATTGTGGGCC-TAMRA | |
| Duox1 forward | AGCCGGGCTGAGTTTGC | XM_587550 |
| Duox1 reverse | AACATGGACTGCACAAACATGTC | |
| Duox1 probe | FAM-AGTCCCTGGGCCTCAAGCCCC-TAMRA | |
| Duox2 forward | GCCCCAGGACATGTTTGTG | Based on conserved sequences between human (NM_014080) and sus scrofa (NM_213999) at the codon level |
| Duox2 reverse | GCCATTGCCATCCTTGTCA | |
| Duox2 probe | FAM-AGTCCATGTTCTCTCTG-MGBNFQ | |
| LPO forward | AAGCAGCGGGACTCTCTACAGA | NM_001009722 |
| LPO reverse | GACCTTCGTGATGTGGGTGTT | |
| LPO probe | FAM-CCTTCTCGCGCCTCA-MGBNFQ |