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. 2009 Oct 27;61(1):41–53. doi: 10.1093/jxb/erp280

Fig. 5.

Fig. 5.

The structure of the GhADF7 gene and construction of GhADF7p:GUS and pGhADF7 expression vectors. (A) GhADF7 gene structure. Exons are denoted by black boxes. Introns, 5′-flanking region and 3′-UTR are denoted by lines. The length of the introns in base pairs are indicated. The number at the boundaries of each exon indicates the codon at which the intron is located. The translation initiation and termination codons are shown. (B) Construction of the chimeric gene between the GhADF7 5′-flanking region and the GUS gene. The length of the 5′-flanking region and cloning sites used for fusion constructs are shown. (C) Construction of the pGhADF7 expression vector containing the GhADF7 coding sequence downstream of its promoter.