Fig. 9.

Overexpression of GhADF7 gene in fission yeast alters cell morphology, leading to the formation of multinucleate cells. (A–D) Cells observed in bright field. (E–H) The same cells observed in dark field. Cells grown in respective conditions were stained with a nucleus-specific fluorescent dye, DAPI. Yeast cells harbouring the GhADF7 gene were induced to undergo longitudinal elongation, resulting in abnormal morphology (A) and the formation of multinucleate cells (E). By contrast, yeast cells harbouring the GhADF7 gene exhibited normal morphology (B) and a single-nucleus (F) in non-induction medium. Likewise, yeast cells harbouring the empty pREP5N vector, grown in induction medium, showed normal morphology (C) and a single-nucleus (G) as those grown under non-induction conditions (D, H). Bar=20 μm. (This figure is available in colour at JXB online.)